NITRO-PAPS

Nitro-PAPS is a highly sensitive colorimetric reagent for Fe(II) detection that forms a water-soluble complex at pH 3.0-8.0 (?max = 582 nm, ? = 107,000). This reagent is suitable for the determination of Fe(II) in serum and can also be used to determine micromolar levels of Cu, Zn, Ni, and Co. Cu and Zn in serum are masked by a mixture of thioglycolic acid and SDS. Zn in serum can be determined by Nitro-PAPS using CN- as a masking reagent of Fe and Cu.
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