Dojindo Dyes Cellular Assays/ Detection-Labeling- kits

Dojindo's company history dates back to the foundation of Dojindo Pharmacie in 1910 by Keji Ueno. Since then Dojindo has been producing for the progress of scientific research.

Today, Dojindo produces over 800 well known products, which are used by research groups worldwide in many disciplines, many of which are the result of collaboration between Dojindo employees and external researchers. Dojindo strives to expand its ability to analyze future development by maintaining a continuous input into new research areas, just as it expands its information gathering capabilities. As we move into the 21st century, Dojindo expects a reduction in production time. Dojindo is ready to meet this shortened production cycle because its creative research heads are working ceaselessly to meet the demands of the future.

Application: NO2 (NO metabolite) detection, fluorometric

Fluorescence detection. Ready-to-use solution

Highly sensitive fluorescence detection. No washing required

Fluorescence detection. Ready-to-use solution

Hoechst dyes are cell membrane permeable and stain DNA to emit intense blue fluorescence. They bind to DNA in the minor groove of poly-AT sequence rich areas. Both Hoechst 33342 and Hoechst 33258 are water-soluble and stable in aqueous solutions. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively.

Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells.

DAPI (4',6-diamidino-2-phenylindole) is a DNA intercalator being specific to AT-rich sequences by attaching the minor groove of the to DNA double helix in the same way as Hoechst dyes do.

Carboxyfluorescein succinimidyl ester For detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy.